DNA purification is a typical and crucial procedure in molecular biology. Purification of DNA is aimed at separating the desired genetic material (chromosomal material) from other contaminants, such as proteins as well as RNA and cell membrane. This is a critical step in almost every molecular application and must be done correctly in order to get high-quality, usable DNA.
There are several different methods for DNA purification. The choice depends on a range of factors such as the starting materials, downstream applications, cost, and time constraints. The common plasmid and genomic purification techniques include chemical treatment, enzyme digestion or mechanical disintegration of tissue/cells followed by salting out of the proteins and precipitating the DNA with alcohol.
Ethanol precipitation can be a low-cost, quick and simple method of desalting and concentrating DNA. DNA molecules clump together in the presence of monovalent cations such as sodium, and then are removed from the solution by using high concentrations of ethanol. This method is employed to remove salts, organic compounds and other impurities. It is often employed with other purification methods.
Anion exchange chromatography is a different popular method of DNA purification. The interaction between negatively charged DNA phosphate backbones as well as the positively-charged surface molecules of resins is what binds DNA in a solvent and positively charged resins. During the binding and washing processes removal of contaminating molecules from the DNA via rigorous wash steps and http://www.mpsciences.com/2021/02/15/science-supplies-for-students/ the DNA that is purified is eluted under low salt conditions.
